Peptide Dosage Calculator – Reconstitution & Dosing
Calculate peptide reconstitution dosing and vial duration
How to Use
- Enter the peptide amount in milligrams (mg) from your vial
- Enter the amount of bacteriostatic water in milliliters (mL)
- Enter your desired dose in micrograms (mcg)
- Enter the frequency of administration (per day or per week)
- Click calculate to see volume per dose and vial duration
What is Peptide Reconstitution?
Peptide reconstitution is the process of mixing lyophilized (freeze-dried) peptide powder with bacteriostatic water to create a liquid solution suitable for injection. This process is essential for peptide therapy as most peptides are shipped in powder form for stability.
Proper reconstitution ensures accurate dosing and maintains peptide stability. The concentration of the final solution depends on the amount of peptide and the volume of bacteriostatic water used.
How to Reconstitute Peptides
- Wash hands thoroughly and use sterile gloves
- Wipe the rubber stopper on both vials with alcohol swabs
- Draw bacteriostatic water into a syringe
- Inject water slowly down the side of the peptide vial (not directly onto the powder)
- Gently swirl (do not shake) until powder is completely dissolved
- Store in refrigerator at 2-8°C (36-46°F)
Calculating Peptide Dosage
To calculate the volume per dose, you need to know the concentration of your reconstituted peptide. The formula is:
Concentration (mcg/mL) = Peptide Amount (mg) × 1000 ÷ Water Volume (mL)
Then, to find the volume per dose:
Volume Per Dose (mL) = Desired Dose (mcg) ÷ Concentration (mcg/mL)
Storage and Stability
Proper storage is crucial for maintaining peptide potency:
- Lyophilized peptides: Store at -20°C (freezer) for long-term storage
- Reconstituted peptides: Store at 2-8°C (refrigerator) for up to 30 days
- Avoid freezing reconstituted peptides unless manufacturer specifies
- Keep away from direct light
- Use within recommended timeframe for optimal potency
- Always check manufacturer guidelines for specific peptides
Safety Considerations
- Always consult with a healthcare provider before starting peptide therapy
- Use only bacteriostatic water, not regular sterile water
- Inspect solution for clarity; discard if cloudy or contains particles
- Never share needles or vials with others
- Dispose of needles and syringes in proper sharps container
- Report any adverse reactions to your healthcare provider immediately
Frequently Asked Questions
- What is the difference between bacteriostatic water and sterile water?
- Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits bacterial growth and allows the reconstituted peptide to remain stable for up to 30 days when refrigerated. Sterile water lacks this preservative and should only be used if the entire vial will be consumed immediately.
- How long does reconstituted peptide last?
- Most reconstituted peptides remain stable for 14-30 days when stored properly in a refrigerator at 2-8°C (36-46°F). However, stability varies by peptide type, so always check manufacturer guidelines and note the date of reconstitution on your vial.
- Can I adjust the amount of bacteriostatic water used?
- Yes, you can adjust the water volume to achieve your desired concentration. Using more water creates a lower concentration, requiring a larger injection volume per dose. Using less water creates a higher concentration, requiring a smaller injection volume. Choose based on your comfort with injection volume and precision needed.
- What if I see particles or cloudiness in my reconstituted peptide?
- Discard any peptide solution that appears cloudy, discolored, or contains visible particles. This may indicate contamination or degradation. Properly reconstituted peptides should be clear and colorless. Always inspect your solution before each use.